[直播課程] 10/2 技術職人_大小鼠輔助生殖技術發展趨勢與基因改造動物之應用

 

Toward a robust infrastructure of mouse and rat resources using improved reproductive technology
Mouse resources are the most established system to deeply understand gene functions in a body and discover innovative technologies to save human lives against diseases. CARD at Kumamoto University has played a role in a hub research center of mouse resources since 1998. To establish the CARD mouse bank, we continuously developed various reproductive technologies and shared the technologies through online manuals and hands-on workshops. In 2012, we co-organized the CARD-NLAC Reproductive Technology Workshop at NLAC in Taiwan. The improved reproductive technology contributes to efficiently managing and sharing mouse resources worldwide and reducing the total number of animals in the research community. Recently, we have been challenged to develop rat reproductive technology and establish a CARD rat bank. In my talk, I will introduce the activities of CARD mouse and rat bank and the related international collaborations.

Toru Takeo, PhD
Professor,
Division of Reproductive Engineering, Center for Animal Resources and Development (CARD), Institute of Resource Development and Analysis, Kumamoto University, Japan

Rat Reproductive Technology
In recent years, genome editing tools have been widely used in rat research. Therefore, the development of efficient production, storage, and transport technologies for genetically modified rats is highly desired. In this talk, I will discuss recent findings and future perspectives on rat reproductive engineering techniques.

Naomi Nakagata, PhD
Professor,
Division of Reproductive Biotechnology and Innovation
Center for Animal Resources and Development (CARD), Institute of Resource Development and Analysis, Kumamoto University, Japan

Recent advancements in mouse reproductive technologies
Capacitation is an important event to achieve fertilization in vivo and in vitro. Recently, we published some papers about in vitro fertilization by selecting capacitated sperm using microfluidic technology, optimized in vivo fertilization by synchronization of the ovulation and copulation timings, and a novel compound (dimethyl-a-cyclodextrin: DMACD) to induce capacitation. These techniques will be applicable to efficiently produce in vitro and in vivo fertilized oocytes of genetically modified mice and contribute to the reduction of animal numbers following the 3Rs principle.

Satohiro Nakao, PhD
Assistant Professor,
Division of Reproductive Engineering, Center for Animal Resources and Development (CARD), Institute of Resource Development and Analysis, Kumamoto University, Japan

The use of database applications in laboratory animal facilities
In the laboratory animal facilities, sharing the most up-to-date information can contribute to improved work efficiency. I’m in charge of system development utilizing information technologies centered on databases at CARD, Kumamoto University. In our system, we manage information on the number of breeding cages, breeding costs, archived mouse strains, and performance of reproductive technologies. Open-source software (Linux, Apache, Tomcat, Java, Spring Boot, and PostgreSQL) and AI (large language models) to assist with coding are used to create robust systems at low cost. The system helps to efficiently manage laboratory animal facilities and mouse bank at CARD.

Shuuji Tsuchiyama
Senior Technical Staff,
Division of Reproductive Engineering, Center for Animal Resources and Development (CARD), Institute of Resource Development and Analysis, Kumamoto University, Japan